Diversidade de Microorganismos: Analysis of fungi and nematodes diversity by classic methods

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Analysis of the diversity of cultivable fungi and oomycote through isolation techniques from soil samples using different culture media. Identification through macroscopic and microscopic analysis of cultures under optical microscopy, in conjugation with methods of molecular analysis, for example amplification of the ITS total region by PCR with subsequent sequencing and BLAST.
Analysis of the nematode diversity by total extraction using the tray method. Identification and quantification of total nematodes by observation under the stereoscopic microscope of the resulting suspensions. Identification based on morphological characteristics. Globodera and Heterodera cysts extraction by soil levigate collection and subsequent quantification.

Keywords: Diversity; Fungi; Nematodes; Classical methods